Penny Boston
Jane Curnutt and I will both be looking at cave interiors
as part of our collaboration on modeling microbial mats. In addition,
I will probably be helping Jut, as needed. If rovers will be there,
it would be nice to try that in the cave. Last year, when the
rover was in the lavatube its battery was not functional so we were
not able to try it. Plus, I plan to provide a Fan Club and Cheering
Section for any tardigrades you may find. What could be more cool and
adorable than these little guys?
Elaine Bryant
I will be collecting soil samples from the 7 original
sampling sites that represent a west (high) to east (low) precipitation
gradient. The samples will then be taken back to the Zzyzx lab used
to inoculate Biolog sole-carbon-source micro plates. This experiment
allows the investigation of community differences as a function of
carbon utilization. DNA extractions of each sample will also
be made for molecular analysis. Given the time, we may be able
to run PCR (polymerase chain reactions) on the samples to begin to
distinguish different species of bacteria, archae and cyanobacteria. Thus,
there will be a day of field work and several days of laboratory work.
Rakesh Mogul
Overall, we are interested in the biochemical and oxidation
properties of the soil. The biochemical properties are the result of microorganisms
that may live in the soil despite the harsh desert conditions of dryness,
temperature, and sun exposure. Related to this, is the degree
of oxidants (molecules that oxidize) in the soil and their impacts
on life in the soil. Thus, we will be conducting simple chemical
experiments to detect the presence of certain enzymes (that are produced
by the microorganisms) and oxidants in the soil in order to address
both the environmental health of the desert and requirements for potential
life-detecting experiments that could be used on Mars and Europa.
Jim Nienow
My group will examine several aspects of the ecophysiology of desert
algae. First, we will look at moisture relations/recovery time
in soil-crust and hypolithic algae communities using pulse-amplitude-modulated
(PAM) fluorescence techniques. Dried samples will be exposed
to set moisture conditions and changes in their fluorescence parameters
monitored over a period of several days. Second, we will investigate
small-scale pattern formation in desert crusts by mapping baseline
fluorescence in rectangular grids. The results will be compared
with values obtained from simple mathematical models of the system. Third,
as time permits, samples of hypolithic and soil-crust associations
will be examined microscopically to determine directly species diversity
and presence.
Henry Sun
In addition to doing microscopy in the field with Dr. Nadeau, we may
also measure the physical strength of desert crusts. We are interested
in the question whether biologically covered soil surfaces are most
resistant to wind erosion. We are not yet certain because the measuring
device is still being made.
Jut Wynne
As part of phase 2 of the Earth-Mars cave detection program,
we have selected the Pisgah lava beds as one of our study sites. My plan
is to deploy sensors once again in several caves (in which we would
likely leave for long term monitoring), collect thermography over a
24 hour period from atop Pisgah crater, and test the ability to communicate
with a rover underground (Mita and I were going to try this at Cima
cave, but the rover’s batteries died and we had to scrub). We
will be testing a new QWIP (thermal IR) camera within the next couple
of months here in Arizona, and I would plan to bring it to the Mojave.
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